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Twist Bioscience mars5
pNoname variant CMV_GFP_BGH after assembly that contains all 6 modules (Promoter region, Coding seqence, <t>MARS5,</t> Terminator and poly adenilation signal, SV40 DTS and the core fragment (bacterial origin of replication and antibiotic resistance gene)
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pNoname variant CMV_GFP_BGH after assembly that contains all 6 modules (Promoter region, Coding seqence, MARS5, Terminator and poly adenilation signal, SV40 DTS and the core fragment (bacterial origin of replication and antibiotic resistance gene)

Journal: bioRxiv

Article Title: Inexpensive and easy method for 6 fragment Golden Gate Assembly of a modular S/MARs mammalian expression vector and its variants

doi: 10.1101/2021.09.04.458594

Figure Lengend Snippet: pNoname variant CMV_GFP_BGH after assembly that contains all 6 modules (Promoter region, Coding seqence, MARS5, Terminator and poly adenilation signal, SV40 DTS and the core fragment (bacterial origin of replication and antibiotic resistance gene)

Article Snippet: Gfp_Kozack (The reporter gene coding for a green fluorescent protein) and MARS5 were synthetized by Twist Biosciences.

Techniques: Variant Assay